IGT® Long-Read DNA Library Prep Kit V2 New Launch

With the rapid development of third-generation sequencing, long-read applications have placed higher demands on library preparation. Whether for complex region analysis, structural variation detection, or long-fragment targeted sequencing, the fragment length, integrity, yield and stability of the library directly affect the subsequent sequencing performance. What the market needs is no longer just a library preparation solution, but long-fragment library products that are longer, more stable and better compatible with third-generation sequencing platforms.

To meet this demand, iGeneTech launches the upgraded IGT® Long-Read DNA Library Prep Kit V2. Equipped with proprietary long-fragment library purification magnetic beads, this kit enables high-quality long-fragment library construction and supports downstream hybridization capture workflows, providing a more efficient and flexible solution for long-read target region research. It better supports third-generation sequencing and fully unlocks the advantages of long-read technology.

Product Advantages

1. Equipped with proprietary long-fragment purification beads, simple and easy workflow

Designed for long-fragment library preparation, this kit balances experimental efficiency and ease of use with a simplified workflow. It effectively reduces operational complexity and errors caused by tedious steps, making long-fragment library construction easier to perform and more reproducible.

Figure 1 Flowchart of library construction using IGT® Long-Read DNA Library Prep Kit V2

2. Average library length up to 6 kb and above

This product delivers libraries with N50 > 8 kb and average sequencing length > 6 kb, which better meets the long-fragment input requirements of third-generation sequencing. It preserves more complete molecular information and provides higher coverage of complex genomic regions.

Figure 2 Library construction using IGT® Long-Read DNA Library Prep Kit V2 with long-fragment library purification by IGT® LongSelect DNA Beads. Sequenced on the ONT platform, the library N50 can reach over 8 kb, with an average fragment length of more than 6 kb.

3. High library yield, sufficient for hybridization capture input

In addition to excellent long-fragment library preparation performance, this solution also achieves outstanding yield. With an average fragment length over 6 kb, the library yield can still reach above 3 μg, balancing long fragments and high output. For hybridization capture experiments, such library input can better meet the requirements of downstream enrichment and expand the application of long-fragment libraries in target region research.

Figure 3 Library construction was performed using IGT® Long-Read DNA Library Prep Kit V2 with long-fragment library purification by IGT® LongSelect DNA Beads.

From Library Preparation to Capture: Expanding Possibilities for Third-Generation Applications

High-quality long-fragment libraries are suitable not only for direct third-generation sequencing but also for various downstream applications such as complex region analysis, structural variation studies and long-fragment target development, broadening the application scope of long-read research.

Proprietary NXT adapter blocking sequence, optimized for long-fragment library capture

To meet the needs of long-fragment library application in hybridization capture, iGeneTech has developed a proprietary NXT adapter blocking sequence based on the structural characteristics of long-fragment libraries. It is better compatible with the hybridization capture system and improves the capture efficiency of long-fragment libraries.

Figure 4 Library construction was performed using IGT® Long-Read DNA Library Prep Kit V2 with long-fragment library purification by IGT® LongSelect DNA Beads.

Capture experiments were conducted using the DMD full-gene probe (target region ~2.2 Mb) combined with Targetseq V3.0 and NXT Blocker.

The capture efficiency reached over 83%, and the 20% X mean depth coverage rate was 98.71%.

Post-capture sequencing N50 reaches 6 kb+

Test results show that the sequencing N50 of captured products can reach above 6 kb, indicating that long-fragment characteristics are well preserved during target enrichment. While enriching target regions, the solution fully exerts the advantages of third-generation sequencing in long reads and complex structure analysis.

Figure 5 The library N50 after capture can reach more than 6 kb, with an average library fragment length of 4.3 kb.

Long-fragment capture: beyond target enrichment

Compared with conventional libraries, long-fragment libraries can not only focus on target regions after capture but also better retain long-range continuous information. This makes them more advantageous in spanning complex regions, resolving repeat sequences, analyzing highly homologous regions and detecting structural variations, helping to obtain more complete and valuable sequencing data.

Figure 6 Data analysis of the custom Panel long‑fragment library after capture revealed significant structural variations.

Conclusion

From long-fragment library construction to downstream capture applications, the requirements for library preparation solutions in third-generation sequencing keep rising: longer libraries, higher yield, smoother workflows and better downstream compatibility. The IGT® Long-Read DNA Library Prep Kit V2 is designed around these practical needs to provide users with a more stable, efficient and scalable long-fragment library preparation solution.

Product Information