Product Introduction
TargetSeq One® Hyb & Wash Kit v3.0 is the fully upgraded TargetSeq One® hybridization and elution kit. This upgrade significantly simplifies reagent composition and operational procedures, reducing the operation time by more than 1 hour. Meanwhile, in terms of product performance, uniformity and capture efficiency have been remarkably improved, reaching an all-new level. This means that no matter when and where the detection is conducted, the results are accurate and reliable, with stable and consistent data.
Product Advantages
01 Only 1 type of Hyb Buffer and 1 type of Wash Buffer
02 Manual operation time takes only 2 hours
03 Excellent performance in uniformity and capture efficiency
04 Outstanding rapid hybridization performance, supporting 30-minute rapid hybridization for pathogens
Workflow
*Ultra-simple kit composition: The kit contains Hyb Human Block, TargetSeq One® Hyb Buffer v3, TargetSeq One® Wash Buffer v3, Post PCR Master Mix, and Post PCR Primer. When combined with Universal Blocking Oligo, Probe, and Cap beads, the experiment can be completed.
Performance
1. TargetSeq One® v3.0 shows high uniformity in capturing different GC regions
Figure 1. TargetSeq One® v3.0, when used in combination with AIExome V5, shows more uniform depth across different GC regions. Compared with competitors, TargetSeq One® v3.0 capture protocol achieves more uniform capture performance in both low-GC and high-GC regions.
2. TargetSeq One® v3.0 exhibits stable performance in rapid hybridization.
TargetSeq One® Hyb & Wash Kit v3.0 supports flexible rapid hybridization protocols, with hybridization time shortened to less than 2 hours and manual operation time reduced to 2 hours, greatly shortening the experimental cycle.
Figure 2. Schematic diagram of time comparison between rapid hybridization and overnight hybridization.
TargetSeq One® Hyb & Wash Kit v3.0 performs excellently in both overnight hybridization and rapid hybridization across different-sized Panels, with stable performance indicators.
Figure 3. Data performance of TargetSeq One® v3.0 hybridization and elution system under different hybridization times. Panels including 2.2 kb (MRD Panel with 32 loci), 438 kb (Solid Tumor Mid Panel), 2.1 Mb (Pan-Cancer Panel V2), and 34.6 Mb (AIExome® Human Exome Panel V5) were used for capture. Overnight hybridization (30 μL hybridization system) and 2-hour rapid hybridization (15 μL hybridization system) were performed respectively, with sequencing conducted on the NovaSeq 6000 platform using PE150.
3. TargetSeq One® v3.0 exhibits outstanding performance in multiple hybridizations
TargetSeq One® Hyb & Wash Kit v3.0, when used in combination with TargetSeq® Eco Universal Blocking Oligo, can block libraries within 6 μg, with excellent performance in capture efficiency and uniformity.
Figure 4. Performance of TargetSeq One® v3.0 hybridization and elution system in combination with TargetSeq® Eco Universal Blocking Oligo in blocking effect for multiplex hybridization reactions. A 2.1 Mb (Pan-Cancer Panel V2) panel was used for capture, with 1-Plex (750 ng)/overnight hybridization, 1-Plex (750 ng)/2-hour rapid hybridization, and 12-Plex (6 μg)/2-hour rapid hybridization performed respectively. Sequencing was conducted on the DNBSEQ T7 platform using PE150.
Product Info
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