iGeneTech Bioscience Co., Ltd.
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Fast Library Prep Kit

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Overview
Advantages
Performance
Ordering Info

Overview

The IGT® Fast Library Prep Kit v2.0 is a rapid library construction kit for fragmented dsDNA. Based on A-T ligation, the kit has a simple workflow and is suitable for input amount ranged from 1 ng to 500 ng.

Advantages

  • Suitable for low input amount and low-quality samples.

  • Simple and rapid workflow, only requires 3.5 h to finish the library construction.

  • Combined with self-developed UDI and UMI technologies, it can effectively reduce data crosstalk and meet the needs of low-frequency variation detection.

Performance

Superior Performance for Low Input Amount Samples

The IGT® Fast Library Prep Kit v2.0 has a high library conversion rate that leads to a higher library yield even with low input amount compared to major competitors.

Superior Performance for Low Input Amount Samples
Excellent Performance for Low-quality Samples

Compared to major competitors, the IGT® Fast Library Prep Kit v2.0 can effectively improve the library quality even with the low-quality samples like FFPE, so as to obtain reliable sequencing results.

Excellent Performance for Low-quality Samples
Higher Molecular Recovery Rate

The IGT® Fast Library Prep Kit v2.0 can effectively reduce library distortion and sequence errors to ensure the high accuracy of the sequencing results.


Compared to major competitors, the kit can obtain 30% to 50% higher sequencing depth after SSCS and DCS.


Higher Molecular Recovery Rate
High Repeatability for Stable Performance

The repeatability test results using the IGT® Fast Library Prep Kit v2.0 showed that the mutation rates detected (VAF ≥ 0.5%) has a superior consistency, indicating the kit is able to achieve accurate and reliable results.

High Repeatability for Stable Performance
High Accuracy for Low-Frequency Mutation Detection

With UMI-UDI adapters, the IGT® Fast Library Prep Kit v2.0 can effectively be used for low-frequency mutation tests.


Tests using reference standard with known 8 mutations with VAF of 0.5% showed that all 8 mutations were successfully detected with highly accurate VAFs.


GeneVariant(AA)Expected allelic frequency(%)Repeat1Repeat2
VafVaf
EGFR
L858R
0.50%
0.40%
0.47%
EGFR
ΔE746-A750
0.50%
0.55%
0.48%
EGFR
T790M
0.50%
0.81%
0.40%
EGFR
V769-D77insASV
0.50%
0.44%
0.25%
KRAS
G12D
0.65%
0.72%
0.70%
KRAS
Q61K
0.65%
0.61%
0.77%
KRAS
A59T
0.65%
0.76%
0.68%
PIK3CA
E545K
0.65%
0.45%
0.70%


Learn more about Fast Library Prep Kit

Ordering Info

Fast Library Prep Kit 2.0
Product NameSetCat. No
IGT® Fast Library Prep Kit v2.016 rxnC10021
96 rxnC10022


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