The IGT® Fast Library Prep Kit v2.0 is a rapid library construction kit for fragmented dsDNA. Based on A-T ligation, the kit has a simple workflow and is suitable for input amount ranged from 1 ng to 500 ng.
Suitable for low input amount and low-quality samples.
Simple and rapid workflow, only requires 3.5 h to finish the library construction.
Combined with self-developed UDI and UMI technologies, it can effectively reduce data crosstalk and meet the needs of low-frequency variation detection.
The IGT® Fast Library Prep Kit v2.0 has a high library conversion rate that leads to a higher library yield even with low input amount compared to major competitors.
Compared to major competitors, the IGT® Fast Library Prep Kit v2.0 can effectively improve the library quality even with the low-quality samples like FFPE, so as to obtain reliable sequencing results.
The IGT® Fast Library Prep Kit v2.0 can effectively reduce library distortion and sequence errors to ensure the high accuracy of the sequencing results.
Compared to major competitors, the kit can obtain 30% to 50% higher sequencing depth after SSCS and DCS.
The repeatability test results using the IGT® Fast Library Prep Kit v2.0 showed that the mutation rates detected (VAF ≥ 0.5%) has a superior consistency, indicating the kit is able to achieve accurate and reliable results.
With UMI-UDI adapters, the IGT® Fast Library Prep Kit v2.0 can effectively be used for low-frequency mutation tests.
Tests using reference standard with known 8 mutations with VAF of 0.5% showed that all 8 mutations were successfully detected with highly accurate VAFs.
Gene | Variant(AA) | Expected allelic frequency(%) | Repeat1 | Repeat2 |
Vaf | Vaf | |||
EGFR | L858R | 0.50% | 0.40% | 0.47% |
EGFR | ΔE746-A750 | 0.50% | 0.55% | 0.48% |
EGFR | T790M | 0.50% | 0.81% | 0.40% |
EGFR | V769-D77insASV | 0.50% | 0.44% | 0.25% |
KRAS | G12D | 0.65% | 0.72% | 0.70% |
KRAS | Q61K | 0.65% | 0.61% | 0.77% |
KRAS | A59T | 0.65% | 0.76% | 0.68% |
PIK3CA | E545K | 0.65% | 0.45% | 0.70% |
Product Name | Set | Cat. No |
IGT® Fast Library Prep Kit v2.0 | 16 rxn | C10021 |
96 rxn | C10022 |
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