As important companion animals to humans, dogs' risks of genetic diseases have long been a concern. Studies have shown that some dog breeds have significant disease susceptibility. For example, the incidence rate of hemangiosarcoma in Golden Retrievers reaches 20%, and the prevalence rate of lymphoma in Boxers is 25%. These data reveal the profound impact of genetic background on dog health. The dog whole exome panel can detect variations in dog coding regions at a relatively low cost, providing an important basis for dog health management and genetic research.
Relying on its independently developed IGT® Oligo Pools synthesis platform and TargetSeq® technology platform, IGT has developed a dog whole exome panel.
IGT dog Whole Exome Panel is designed based on the 36.0Mb CDS region of the canFam4 reference genome, with a total of 1,000,193 probes. Through rigorous screening and optimization, it enables efficient and uniform capture of the CDS regions of dogs. Using dog gDNA for sequencing on the MGI platform, the capture efficiency exceeds 80%, the 20× uniformity reaches over 95%, and with only 8Gb of data volume, a sequencing depth of over 110× can be achieved, boasting excellent performance indicators.
Figure 1. Target mean depth of the Dog Whole Exome Panel
Figure 2. Performance of Effective Depth of the Dog Whole Exome Panel at 8 Gb Data Volume
Product Applications
01 Screening and Diagnosis of Hereditary Diseases:
For common hereditary diseases in dogs (such as hip dysplasia, progressive retinal atrophy, etc.), whole exome sequencing can accurately locate pathogenic gene variants. This assists veterinarians in confirming diagnoses for dogs suspected of illness, or conducting early risk assessments for healthy dogs, enabling early intervention to reduce the probability of onset.
02 Breeding Optimization and Breed Improvement:
In kennel breeding, the dog whole exome sequencing is used to screen individuals with favorable genes and no harmful mutations as breeding parents. This reduces the transmission of hereditary diseases in offspring, while also allowing targeted enhancement of breed characteristics (such as genes related to body type and temperament), improving breeding efficiency and the quality of offspring.
03 Research on Rare Diseases and Complex Diseases:
For rare hereditary diseases or complex diseases with unknown causes in dogs (such as immunodeficiency, metabolic abnormalities, etc.), whole exome sequencing can quickly identify potential pathogenic genes. This provides insights for studying disease pathogenesis, developing precision treatment plans, and advancing veterinary research on dogs.
04 Personalized Health Management for Pets:
Provide pet owners with genetic background analysis of dogs, including information on disease-susceptible genes and drug response-related genes. This helps develop personalized diet, care, and medical plans, improving pets' healthy lifespan and quality of life.
As of now, IGT has launched over ten animal and plant whole exome panels, with more panels under development. All products support customization and semi-customization.
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