From genetic testing to oncology targeted research and pathogen capture, the applications of NGS targeted enrichment continue to expand, placing higher demands on experimental efficiency and data quality. How to shorten hybridization waiting time while maintaining capture efficiency and uniformity has become a key concern for laboratories optimizing their workflows.
To meet these needs, iGeneTech has launched the SnapHyb® Hyb & Wash Kit (hereinafter referred to as SnapHyb®), supporting flexible hybridization from 1–30 min, with hybridization in as little as 1 min. Combined with a simpler reagent composition and workflow, it provides an efficient choice for applications such as whole-exome capture, precision oncology testing, and pathogen detection.
Highlight 1: Hybridization in as Little as 1 min for More Flexible Scheduling
SnapHyb® offers hybridization times from 1–30 min, allowing laboratories to arrange experiments flexibly according to project requirements: 1 min and 5 min ultra-fast hybridization suit time-critical projects, while the 30 min option is available for projects requiring more thorough capture.
In tests with the Pan-Cancer Panel V2 and NSCLC Panel V2, SnapHyb® delivered good capture efficiency and uniformity at 1, 5, and 30 min of hybridization. By adjusting hybridization time, laboratories can evaluate parameters suited to their own projects within a single reagent system, establishing more flexible workflows for rapid turnaround and routine testing research.
Pan-Cancer Panel V2 Performance Metrics
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NSCLC Panel V2 Performance Metrics
Figure 1: Capture performance at 1, 5, and 30 min hybridization times. Using gDNA reference standard (Promega, G3041) as sample, 500 ng input per library, captured with Pan-Cancer Panel V2 (2.1 Mb) and NSCLC Panel V2 (170 Kb) respectively under 1, 5, and 30 min hybridization conditions, sequenced on the GeneMind SURFSeq 5000 platform with PE150.
Highlight 2: Simpler Reagent Composition for a Smoother Workflow
Faster experiments come not only from shorter hybridization times but also from simplified reagent preparation and manual handling. SnapHyb® hybridization setup requires only Hyb Mix and probes, with no need to add Human Cot-1 DNA or Adapter block, reducing reagent preparation steps and operational complexity.
In terms of workflow design, SnapHyb® connects library concentration, hybridization, bead binding, washing, and amplification/purification steps. Compared with conventional rapid hybridization workflows, it requires less reagent preparation and fewer washing steps, helping technicians schedule operations with greater ease and extending the benefits of rapid hybridization into daily routines.
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Figure 2: Schematic workflow of the SnapHyb® Hyb & Wash Kit.
Highlight 3: Excellent Whole-Exome Capture Performance, Balancing Uniformity and Variant Detection
Whole-exome sequencing covers broad regions, so capture uniformity and variant detection performance are important dimensions for evaluating a solution. Paired with the AIExome® Human Exome Panel V5 (core exome) and the AIExome® Human Medical Exome (medical exome), SnapHyb® achieved good capture performance under 30 min hybridization, providing a high-quality data foundation for genetics-related research.
In NA12878 reference standard testing, the core exome with SnapHyb® achieved overall Recall, Precision, and F1 score above 99%, with SNP and InDel detection also showing good performance. From capture metrics to variant analysis results, SnapHyb® offers a superior choice for whole-exome capture sequencing, balancing efficiency and data quality.
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Figure 3: Capture performance of the core exome and medical exome, and NA12878 variant detection results. Capture performance testing used gDNA reference standard (Promega, G3041) as sample, 500 ng input per library, captured with the core exome and medical exome Panels, 30 min hybridization. Variant detection testing used the NA12878 reference standard, captured with the core exome Panel, 30 min hybridization; reference variants and high-confidence regions are from GIAB NIST v3.3.2. Sequencing was performed on the GeneMind SURFSeq 5000 platform with PE150.
Highlight 4: Expanding Pathogen Capture Applications with Good Coverage at 5 min Hybridization
Pathogen capture requires attention to enrichment performance across samples of different loads, as well as completeness of target genome coverage. With the Influenza A/B/C Virus Panel, SnapHyb® was tested with 5 min hybridization on influenza virus positive samples of varying loads. Both high-load and low-load samples showed good genome coverage, demonstrating the potential of rapid hybridization in whole-genome pathogen capture.
Figure 4: Capture results for influenza virus samples of different loads. Influenza virus positive samples of varying loads were captured with the Influenza A/B/C Virus Panel, using SnapHyb® with 5 min hybridization, sequenced on the GeneMind SURFSeq 5000 platform with PE150.
For research projects such as pathogen full-length capture and tNGS, shortening hybridization waiting time provides more room for workflow optimization. Validation with actual samples and target Panels also helps establish rapid capture solutions suited to project needs.
Highlight 5: Compatible with Multiple Probe Types, Adapting Rapid Hybridization to More Projects
Beyond application scenarios, compatibility with probe types and sequencing platforms is a key consideration when laboratories choose a hybrid capture solution. SnapHyb® is recommended with TargetSeq® DNA Probes HT probes, is also compatible with column-synthesized DNA probes, and works with Illumina, MGI, GeneMind, and other sequencing platforms, facilitating workflow evaluation and application across different technology systems.
In tests with column-synthesized competitor Panel and CNV Backbone Panel, SnapHyb® achieved good capture efficiency and uniformity. In particular, data from the CNV Backbone Panel at 2, 5, 15, and 30 min hybridization conditions further demonstrate its adaptability to different time schemes, providing a reference for introducing rapid hybridization into existing probe systems.
Figure 5: Performance with column-synthesized DNA probes. Top: capture performance of a competitor Exome v2 Panel under different hybrid capture solutions; Bottom: capture performance of a competitor CNV Backbone Panel with SnapHyb® at 2, 5, 15, and 30 min hybridization.
From 1 min ultra-fast hybridization to flexible 30 min schemes, from whole-exome and oncology Panels to pathogen capture, SnapHyb® brings a more efficient experimental choice for NGS targeted enrichment with its simpler reagent composition and multi-scenario performance.
Ordering Information
Recommended pairing:
TargetSeq® DNA Probes HT
TargetSeq® Cap Beads & Nuclease-Free Water
Contact your iGeneTech sales representative for product details and testing solutions.
For research use only. Not for use in clinical diagnosis.
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