Hyb & Wash Kit

Figure 1. TargetSeq One® Hyb & Wash Kit v3.0, when combined with AIExome V5, exhibits more uniform depth performance across different GC-content regions. Compared with competitors, the TargetSeq One® v3.0 capture protocol can achieve more uniform capture performance in both low-GC and high-GC regions.

TargetSeq One® Hyb & Wash Kit v3.0 supports flexible rapid hybridization protocols, with hybridization time reducible to less than 2 hours and manual operation time cut down to 2 hours, which significantly shortens the overall experimental cycle.

Figure 2. Schematic diagram of time comparison between rapid hybridization and overnight hybridization.

TargetSeq One® Hyb & Wash Kit v3.0 delivers excellent performance and stable performance metrics in rapid hybridization for panels of different sizes.

Figure 3. Data performance of TargetSeq One® Hyb & Wash Kit v3.0 across different hybridization durations. Capture experiments were performed using panels of 2.2 kb (MRD Panel with 32 loci), 438 kb (Solid Tumor Mid Panel), 2.1 Mb (Pan-Cancer Panel V2), and 34.6 Mb (AIExome® Human Exome Panel V5). Overnight hybridization (30μL hybridization system) and 2-hour rapid hybridization (15μL hybridization system) were conducted respectively, followed by PE150 sequencing on the NovaSeq 6000 platform.

TargetSeq One® Hyb & Wash Kit v3.0, when combined with TargetSeq® Eco Universal Blocking Oligo, can block libraries of up to 6μg, while delivering excellent capture efficiency and uniformity.

Figure 4. Blocking performance of TargetSeq One® Hyb & Wash Kit v3.0 combined with TargetSeq® Eco Universal Blocking Oligo in multiplex hybridization reactions. Capture was performed using a 2.1 Mb (Pan-Cancer Panel V2) panel, with 1-Plex (750 ng)/overnight hybridization, 1-Plex (750 ng)/2-hour rapid hybridization, and 12-Plex (6μg)/2-hour rapid hybridization conducted separately, followed by PE150 sequencing on the DNBSEQ T7 platform.