iGeneTech Bioscience Co., Ltd.
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RNA Exome

Overview
Product Specifications
Advantages
Performance
Ordering Info
Resources

Overview

Comprehensively covers the CDS regions of the whole transcriptome, incorporates key UTR regions related to fusions after in-depth screening, and adds edge probes on both sides of exons to ensure the capture capability of exon boundaries. Additionally, it is engineered at the design level to eliminate rRNA and globin sequences, reducing redundant data and improving utilization efficiency.

It retains the high sensitivity and efficiency of targeted enrichment while possessing the discovery capability of whole transcriptome sequencing, enabling accurate targeting of key transcripts. It assists researchers in in-depth exploration of gene transcription mechanisms and holds significant application value in fields such as cancer gene fusion detection and research on pathogenic mechanisms of rare diseases.


Learn More About RNA Exome

Product Specifications

Product Specifications

Technical Platform

TargetSeq® Hybridization Capture Sequencing

Coverage Size

46.3 Mb

Reference Database

RefSeq

Reference Genome

GRCh37 (hg19)

Coverage

Whole transcriptome CDS regions and fusion-related UTR regions

Storage

-20℃±5℃

Sequence Platform

Illumina / MGI

Recommend Sequencing Read Length

PE150

Recommend Sequencing Data Size

3 Gb


Performance

Excellent Performance

Accurate_detection_of_fusions.jpg


Figure 1. Capture Performance Test of RNA Standards. Shuimu Jiheng RNA standards were used to construct libraries with input amounts of 10 ng and 200 ng using RNA library construction kits. After hybrid capture, they were sequenced on Illumina NovaSeq 6000 (PE150).


Accurate detection of fusions


Table 1. Verification Results of Fusion Standards

Fusion Name

10 ng Input  
Supported Reads

200 ng Input
Supported Reads

ETV6--NTRK3

91.5

115

ETV6--NTRK3

76.5

108.5

PAN3--NTRK2

38

32.5

ETV6--NTRK3

67

94.5

ETV6--NTRK3

80.5

89

LMNA--NTRK1

32

41

TRIM24--NTRK2

12.5

20

SDC4--ROS1

15.5

19.5

SQSTM1--NTRK1

17.5

14

TPM3--NTRK1

16

25.5

NACC2--NTRK2

15.5

23

CCDC6--RET

13

11

QKI--NTRK2

14

20.5

TFG--NTRK1

12

20

AFAP1--NTRK2

9.5

21

IRF2BP2--NTRK1

13

12

BTBD1--NTRK3

16

46

CD74--ROS1

7.5

15

SDC4--ROS1

8.5

16

CD74--ROS1

13.5

17

EML4--ALK

5.5

9

EML4--ALK

5.5

7

SLC34A2--ROS1

5.5

11

Note: The results of the comparative analysis of the detection of fusion events in positive standards show that under the condition of the same data volume (~8 Gb) and different input amounts, all fusion events were detected, consistent with the expected results.


Ordering Info

RNA Exome

Product Name

Panel Number

Set

Cat. No

RNA Exome

T2582V1


24 rxn

PH2007125

96 rxn

PH2007122




Resources

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