iGeneTech Bioscience Co., Ltd.
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Pan-Cancer Panel

Overview
Performance
Ordering Info
Resources

Overview

Based on TargetSeq® hybrid capture sequencing technology, it covers 641 genes related to targeted therapy for solid tumors and tumor genetic susceptibility. Additionally, it includes intronic regions of 38 gene hot spot fusions, 15 classic microsatellite loci, and 219 chemotherapy-related loci. It enables one-time detection of variant types such as point mutations, fusions, copy number variations, and insertions/deletions, as well as TMB and MSI analyses, providing references for tumor targeted therapy, immunotherapy, and risk prediction of hereditary tumors, among others.

Pan-Cancer-Panel

Product Parameter

Panel Number

T893V1

Technical Platform
TargetSeq® Hybridization Capture Sequencing
Coverage Size
2.1 Mb
Reference Database
RefSeq
Reference Genome
GRCh37 (hg19)
Coverage
641 genes, and additionally includes intronic regions of 38 gene hot spot fusions, 15 classic microsatellite loci, and 219 chemotherapy-related loci
Storage

-20℃±5℃

Sequence Platform
Illumina / MGI
Recommend Sequencing Read Length
PE150
Recommend Sequencing Data Size and Depth
3Gb / 500×


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Advantages

  • Comprehensive coverage of solid tumor-associated genes.

  • Various types of mutations including MSIs and fusions, and TMB and MSI calculation available.

  • Proven TargetSeq One® workflow provides simple and reliable results.

  • Semi-customization applicable.

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Performance

Excellent Performance

pan1.png

Figure 1. Capture Data Performance Test of gDNA Standards.

gDNA positive standards (Jingliang, cat. GW-OGTM800) were used to construct libraries with an input amount of 50 ng, followed by hybrid capture with Pan-Cancer Panel V2 and sequencing on Illumina NovaSeq 6000 (PE150).


High Sensitivity & Accuracy

Table 1. Results of gDNA reference standard

Gene

Variant

Expected allelic frequency

Reported allelic frequency

EGFR

L858R

1.0%

0.9%

KRAS

A146T

1.0%

1.4%

NRAS

Q61K

1.0%

0.9%

EGFR

T790M

2.0%

1.9%

EGFR

E746_A750del

2.0%

1.5%

FLT3

I836del

2.0%

1.8%

KIT

D816V

2.0%

2.3%

KRAS

G12D

2.0%

1.6%

EGFR

A767_V769dup

3.0%

1.9%

EGFR

G719S

4.0%

4.5%

KRAS

G13D

4.0%

3.3%

EML4-ALK

Fusion

5.0%

3.6%

CD74-ROS1

Fusion

6.0%

2.1%

BRAF

V600E

7.0%

4.7%

PIK3CA

H1047R

7.0%

7.1%

Note: Results of comparative analysis on the detection of mutation sites in positive standards. gDNA standards (Jingliang, cat. GW-OPSM003) were used to construct libraries with an input amount of 50 ng, followed by sequencing on Illumina NovaSeq 6000 (PE150). After deduplication, when the depth reached ~1,000× (7 Gb raw bases), all positive mutation sites were detected, consistent with the expected results.


UMI for Low-frequency Mutations

UMI with cfDNA liquid biopsy for low-frequency mutation detection has shown lower background noises and thus more accurate results. By using cfDNA reference standard (Horizon, #HD780) , the results showed all 8 mutations with 0.5% mutation rate can be detected precisely as expected.


Table 2. Results of cfDNA reference standard

GeneVariantExpected Allelic FrequencyReported allelic frequency
EGFR
L858R
0.50%
0.19%
EGFR
T790M
0.50%
0.35%
EGFR
ΔE746_A750
0.50%
0.27%
KRAS
A146T
0.50%
0.23%
KRAS
G12D
0.50%
0.64%
KRAS
G13D
0.50%
0.76%
NRAS
Q61K
0.50%
0.44%
PIK3CA
E545K
0.50%
0.44%


Learn More About Pan-Cancer Panel

Ordering Info

TargetSeq® Pan-Cancer Panel V2
Product NamePanel NumberSetCat. No

TargetSeq® Pan-Cancer Panel V2

T893V1

24 rxn

PT1011715

96 rxn

PT1011712


Resources

Demo DataSet
  • TargetSeq® Pan-Cancer Panel V2 (T893V1) demo data
    TargetSeq® Pan-Cancer Panel V2 (T893V1) demo data
bed File
  • TargetSeq® Pan-Cancer Panel V2 (T893V1) bed file
    TargetSeq® Pan-Cancer Panel V2 (T893V1) bed file
Flyer
  • Pan-cancer Panel V2
    Pan-cancer Panel V2