TargetSeq® Solid Tumor Mid Panel

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Overview

TargetSeq® Solid Tumor Mid Panel covers 122 gene coding regions related to solid tumors, 10 hotspot fusion gene intron regions, 19 classic microsatellite sites, and 219 chemotherapy-related sites.

Product Parameter

Panel Number	T1288V1
Technical Platform	TargetSeq® Hybridization Capture Sequencing
Coverage Size	438.3 kb
Reference Database	RefSeq
Reference Genome	GRCh37 (hg19)
Coverage	CDS region of 122 genes, 10 hotspot fusion gene intron regions, 19 MSI and 219 chemotherapy-related sites.
Storage	-20℃±5℃
Sequence Platform	Illumina / MGI
Recommend Sequencing Read Length	PE150
Recommend Sequencing Data Size and Depth	1.5Gb/1,000X

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Advantages

Selected coverage of 122 key pan-cancer genes;
Simultaneous detection of various variant types including SNP/Indel, CNV, Fusion, and MSI.
Coupled with high-efficiency library preparation and hybrid capture reagents, it accurately targets low-abundance variants.

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Performance

Capture Performance Test

Figure 1. Test of Capture Data Performance of gDNA Reference Standards. gDNA positive reference standards (Shuimu Jiheng, PSC500) were used to construct libraries with an input amount of 100 ng, followed by hybrid capture and sequencing on the Illumina NovaSeq 6000 with PE150.

Mutation Detection

Table 1. Detection results of mutation sites in gDNA positive reference standards

Gene	Variant	Expected Allelic Frequency	Reported Allelic Frequency
EGFR	L858R	1.0%	0.9%
KRAS	A146T	1.0%	1.4%
NRAS	Q61K	1.0%	0.9%
EGFR	T790M	2.0%	1.9%
EGFR	E746_A750del	2.0%	1.5%
FLT3	I836del	2.0%	1.8%
KIT	D816V	2.0%	2.3%
KRAS	G12D	2.0%	1.6%
EGFR	A767_V769dup	3.0%	1.9%
EGFR	G719S	4.0%	4.5%
EML4-ALK	Fusion	5.0%	3.6%

Note: Results of comparative analysis on detection of mutation sites in positive standards. Shuimu Jiheng gDNA and cfDNA positive standards were used for testing, with sequencing performed on the Illumina NovaSeq 6000 platform (PE150). All positive mutation sites were detected, which was consistent with the expected results.

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Ordering Info

TargetSeq® Solid Tumor Mid Panel

Product Name	Panel Number	Set	Cat. No
TargetSeq® Solid Tumor Mid Panel	T1288V1	16 rxn	PH2002101
TargetSeq® Solid Tumor Mid Panel	T1288V1	96 rxn	PH2002102

Resources

Demo DataSet

TargetSeq® Solid Tumor Mid Panel (T1288V1) demo data
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bed File

TargetSeq® Solid Tumor Mid Panel (T1288V1) bed file
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Articles

Cross-oncopanel study reveals high sensitivity and accuracy with overall analytical performance depending on genomic regions

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Binsheng Gong; Dan Li; Rebecca Kusko; Natalia Novoradovskaya; Yifan Zhang; Shangzi Wang; Carlos Pabón-Peña; Zhihong Zhang; Kevin Lai; Wanshi Cai; Jennifer S. LoCoco; Eric Lader; Todd A. Richmond; Vinay K. Mittal; Liang-Chun Liu; Donald J. Johann; James C. Willey; Pierre R. Bushel; Ying Yu; Chang Xu; Guangchun Chen; Daniel Burgess; Simon Cawley; Kristina Giorda; Nathan Haseley; Fujun Qiu; Katherine Wilkins; Hanane Arib; Claire Attwooll; Kevin Babson; Longlong Bao; Wenjun Bao; Anne Bergstrom Lucas; Hunter Best; Ambica Bhandari; Halil Bisgin; James Blackburn; Thomas M. Blomquist; Lisa Boardman; Blake Burgher; Daniel J. Butler; Chia-Jung Chang; Alka Chaubey; Tao Chen; Marco Chierici; Christopher R. Chin; Devin Close; Jeffrey Conroy; Jessica Cooley Coleman; Daniel J. Craig; Erin Crawford; Angela del Pozo; Ira W. Deveson; Daniel Duncan; Agda Karina Eterovic; Xiaohui Fan; Jonathan Foox; Cesare Furlanello; Abhisek Ghosal; Sean Glenn; Meijian Guan; Christine Haag; Xinyi Hang; Scott Happe; Britt

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Genome Biology 2021;22(1):109 DOI:10.1186/s13059-021-02315-0

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Ultra-deep multi-oncopanel sequencing of benchmarking samples with a wide range of variant allele frequencies

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Binsheng Gong; Rebecca Kusko; Wendell Jones; Weida Tong; Joshua Xu

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Scientific Data 2022;9(1):288 DOI:10.1038/s41597-022-01359-6

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