CNOverview
The FLT3 internal tandem duplication (ITD) mutation is one of the most common pathogenic mutations in acute myeloid leukemia (AML), playing a crucial role in prognosis and targeted therapy guidance for hematological malignancies. To address this, our approach utilizes multiplex amplicon sequencing technology combined with a unique primer design strategy, incorporating the entire region from intron 13 to exon 15 into the target area. By enabling random amplification, this method enhances detection sensitivity to accurately identify both known and unknown ITD mutations occurring in exons 14-15.
