iGeneTech Bioscience Co., Ltd.
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MultipSeq® BRCA1/2 Research Assay V2

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Overview
Advantages
Performance
Ordering Info
Resources

Overview

Coverage Size19.5 kb
CoverageIncludes all exon regions of BRCA1 & BRCA2 genes, at least 10 bp of upstream and downstream flanking regions, and UTR regions, as well as 228 pathogenic, likely pathogenic, and variants of uncertain significance located in non-exon regions from the ClinVar database.
Detection RangeSNV, InDel
Recommend Sequencing Data0.2 Gb/5000×
ApplicationsHealth screening and risk assessment




Advantages

Highly optimized workflow

Based on self-developed MultipSeq® multiplex amplicon sequencing technology, enabling highly specific targeted enrichment.

Comprehensive coverage

Fully covers the regions to be tested.

Excellent performance

Stable and superior metrics in coverage, capture efficiency, and uniformity.

Convenient and efficient

Short experimental cycle (3-4 hours) and simple operation.

Performance

Excellent Performance

Capture performance remains consistently stable and superior across various sample types


 Performance_01.jpg


Performance evaluation of the MultipSeq® BRCA1/2 Research Assay V2 (for Illumina) kit across different sample types.

Genomic DNA (gDNA) was extracted from blood samples, female saliva samples, and male saliva samples, with 40 ng of each input for library preparation. Sequencing was performed on the NovaSeq 6000 platform using PE150 read length, generating approximately 120 Mb of sequencing data.

 

High sensitivity and accuracy in mutation detection

Reference material mutation detection results


Performance_02.png 


Note: iGeneTech MultipSeq® BRCA1/2 Research Assay V2 (for Illumina) was used to test Cobio BRCA reference materials: the detection rate for 21 loci was 100%, and the detected frequencies were essentially consistent with the theoretical mutation frequencies.


Learn More About MultipSeq® BRCA1/2 Research Assay V2

Ordering Info

MultipSeq® BRCA1/2 Research Assay V2
Product NameSetCat. No
MultipSeq® BRCA1/2 Research Assay V2 (for Illumina)4 rxnM62230
24 rxnM62235
96 rxnM62232
MultipSeq® BRCA1/2 Research Assay V2 (for MGI PE100)4 rxnM62240
24 rxnM62245
96 rxnM62242



Resources

bed File
  • MultipSeq® BRCA1/2 Research Assay V2 bed file
    MultipSeq® BRCA1/2 Research Assay V2 bed file
Demo DataSet
  • MultipSeq® BRCA1/2 Research Assay V2 demo data
    MultipSeq® BRCA1/2 Research Assay V2 demo data