Coverage Size | 19.5 kb |
Coverage | Includes all exon regions of BRCA1 & BRCA2 genes, at least 10 bp of upstream and downstream flanking regions, and UTR regions, as well as 228 pathogenic, likely pathogenic, and variants of uncertain significance located in non-exon regions from the ClinVar database. |
Detection Range | SNV, InDel |
Recommend Sequencing Data | 0.2 Gb/5000× |
Applications | Health screening and risk assessment |
Based on self-developed MultipSeq® multiplex amplicon sequencing technology, enabling highly specific targeted enrichment.
Fully covers the regions to be tested.
Stable and superior metrics in coverage, capture efficiency, and uniformity.
Short experimental cycle (3-4 hours) and simple operation.
Capture performance remains consistently stable and superior across various sample types
Performance evaluation of the MultipSeq® BRCA1/2 Research Assay V2 (for Illumina) kit across different sample types.
Genomic DNA (gDNA) was extracted from blood samples, female saliva samples, and male saliva samples, with 40 ng of each input for library preparation. Sequencing was performed on the NovaSeq 6000 platform using PE150 read length, generating approximately 120 Mb of sequencing data.
High sensitivity and accuracy in mutation detection
Reference material mutation detection results
Note: iGeneTech MultipSeq® BRCA1/2 Research Assay V2 (for Illumina) was used to test Cobio BRCA reference materials: the detection rate for 21 loci was 100%, and the detected frequencies were essentially consistent with the theoretical mutation frequencies.
Product Name | Set | Cat. No |
MultipSeq® BRCA1/2 Research Assay V2 (for Illumina) | 4 rxn | M62230 |
24 rxn | M62235 | |
96 rxn | M62232 | |
MultipSeq® BRCA1/2 Research Assay V2 (for MGI PE100) | 4 rxn | M62240 |
24 rxn | M62245 | |
96 rxn | M62242 |