Oligo Pools - iGeneTech Bioscience Co., Ltd.

Overview

Advantages

Performance

Overview

IGT® Oligo Pools is a single-stranded oligonucleotide pool product synthesized based on iGeneTech's independently developed Ignite 3.0 high-throughput synthesis platform.

In situ synthesized IGT® Oligo Pools feature high uniformity and high yield. The synthesized Oligo Pools dry powder can be used for sgRNA library preparation and DNA data storage. Combined with in vitro amplification and biotin labeling, it can also be applied to scenarios such as NGS capture probe preparation.

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Advantages

High Throughput

Synthesizes 680,000 independent sites per run, meeting the needs of WES probe libraries, large-scale genetic screening, and other applications.

Short Turnaround Time

Delivery of Oligo Pools dry powder takes only 3–5 working days (specific time depends on sequence length), and ready-to-use probes take only 10–15 working days. It accelerates R&D cycles and facilitates efficient project progress.

High Quality

Stably supports 200nt. The average single-base error rate (mismatch + insertion/deletion) is <0.2%. The average yield per Oligo is >0.5 fmol, and the 95th percentile/5th percentile ratio (95/5) is <4. The synthesized sequence coverage rate is >99.9%, ensuring library integrity.

End-to-End Solution

For hybridization capture applications, it provides professional probe design, Oligo Pools synthesis, ready-to-use probes, and full-process reagents for extraction, library construction, and capture.

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Performance

Excellent Performance

IGT® Oligo Pools achieves full coverage and high uniformity of target sequences, with an error rate of less than 0.2%.

Figure 1. IGT® Oligo Pools exhibits excellent uniformity.

A 200nt oligonucleotide pool containing 30,000 unique sequences was synthesized, amplified, and subjected to next-generation sequencing (NGS). The sequencing depth distribution of each oligonucleotide in the pool was analyzed by counting the number of sequencing reads corresponding to each unique sequence.

The results show that it has excellent uniformity (95th/5th percentile ratio = 1.82), ensuring that each sequence is equally sampled in downstream screening.

Figure 2. IGT® Oligo Pools features a low error rate.

Oligonucleotides of 130nt with 3,000 unique sequences were synthesized and amplified, with unique molecular identifiers (UMIs) added prior to amplification. After amplification, next-generation sequencing (NGS) was performed. Consensus sequences were generated by clustering sequencing reads carrying the same UMI, and then aligned with the original designed sequences.

Detection results show that the comprehensive error rate is only 0.1629% (including base substitutions, insertions, and deletions), which is much lower than the industry average.

Probes prepared with IGT® Oligo Pools deliver highly uniform capture data.

Figure 3. Capture Data Performance of Probes Prepared with IGT® Oligo Pools

Panels of 42.5kb and 2.6Mb were used for capture. The capture data showed high uniformity across different GC content regions.