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Two-step fitness selection for intra-host variations in SARS-CoV-2

分类：文献
作者：
来源：
发布时间：2019-03-18
访问量：0

【概要描述】捕获效率(CaptureRate)：表示有效数据的比例，越高越好，捕获效率越高的话，意味着达到变异检测需要的RawData数据量越少。需要注意的是：捕获效率和均一度这两个指标是互斥的，因为要保证目标区域上边界区的均一度，在探针设计的时候就要适当往外做延伸，这样会多测到旁边的非目标区域，会使捕获效率降低。

Two-step fitness selection for intra-host variations in SARS-CoV-2

分类：文献
作者：
来源：
发布时间：2019-03-18
访问量：0

详情

> Cell Reports 2022 Jan 11;38(2):110205. doi: 10.1016/j.celrep.2021.110205. Epub 2021 Dec 16

Two-step fitness selection for intra-host variations in SARS-CoV-2

Jiarui Li, Pengcheng Du, Lijiang Yang, Ju Zhang, Chuan Song, Danying Chen, Yangzi Song, Nan Ding, Mingxi Hua, Kai Han, Rui Song, Wen Xie, Zhihai Chen, Xianbo Wang, Jingyuan Liu, Yanli Xu, Guiju Gao, Qi Wang, Lin Pu, Lin Di, Jie Li, Jinglin Yue, Junyan Han, Xuesen Zhao, Yonghong Yan, Fengting Yu, Angela R. Wu, Fujie Zhang, Yi Qin Gao, Yanyi Huang, Jianbin Wang, Hui Zeng, Chen Chen

DOI：10.1016/j.celrep.2021.110205

Abstract
Spontaneous mutations introduce uncertainty into coronavirus disease 2019 (COVID-19) control procedures and vaccine development. Here, we perform a spatiotemporal analysis on intra-host single-nucleotide variants (iSNVs) in 402 clinical samples from 170 affected individuals, which reveals an increase in genetic diversity over time after symptom onset in individuals. Nonsynonymous mutations are overrepresented in the pool of iSNVs but underrepresented at the single-nucleotide polymorphism (SNP) level, suggesting a two-step fitness selection process: a large number of nonsynonymous substitutions are generated in the host (positive selection), and these substitutions tend to be unfixed as SNPs in the population (negative selection). Dynamic iSNV changes in subpopulations with different gender, age, illness severity, and viral shedding time displayed a varied fitness selection process among populations. Our study highlights that iSNVs provide a mutational pool shaping the rapid global evolution of the virus.

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上一个: PCR Primer Design for the Rapidly Evolving SARS-CoV-2 Genome

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上一个: PCR Primer Design for the Rapidly Evolving SARS-CoV-2 Genome

下一个: 无